Misprocessed tRNA response targets piRNA clusters.

Germline PIWI-interacting RNA (piRNA) clusters have a characteristic transcriptional status; although they do not have their own promoters, both genomic strands are transcribed, while splicing and 30 processing signals are neglected. How this transcription is maintained remains unknown. Molla-Herman et al (2015) discovered that mutations in a tRNA processing factor cause the loss of transcription at some piRNA clusters, leading to sterility in Drosophila melanogaster. This defect in piRNA cluster transcription is restored by mutations in genes required for the DNA damage checkpoint or replication.